WebDec 16, 2024 · Cyto-GCaMP6f fluorescence is localized to Müller glia and a subset of neurons. Scale bar: 10 µm. Right, average projection of IPL in Slc1a3-CreER;Lck … WebLck-GCaMP6f is a detector of single neuronal action potentials with fast response kinetics. It has an Lck-tag to improve its ability to reveal calcium signals in near membrane regions and fine processes ( e.g. , astrocyte processes). After Cre exposure, bright GCaMP6f fluorescence is observed following calcium binding (such as neuronal ...
Brain-state dependent astrocytic Ca2+ signals are coupled to both ...
WebThese results clearly demonstrated that the GCaMP6f-mediated Ca 2+ signal from either neurons or astrocytes is specifically detectable in vivo via optical fiber, showing distinct temporal features to sensory stimulation. WebDec 16, 2024 · CRITICAL: For calcium imaging experiments, a mix of both viral constructions (AAV2.5-gfaABC1D-cyto-GCaMP6f (viral titer range 2–6 × 10 13 vg/ml) and AVV2.5-GFAP-Cre-mCherry (4.3 × 10 12 vg/ml; 1:1 ratio) are injected. c. Place the tip of the needle in the first position and make a mark scratching softly on the surface of the skull … how to save the hand of the inheritor
Astrocytes mediate migraine-related intracranial meningeal …
WebDec 16, 2024 · To verify loss of calcium transients in neurons and Müller glia following BAPTA-AM application, we include imaging results from PDGFRα;cyto-GCaMP6f flox mice (PDGFRα-Cre: strain 013148), which label Müller glia and a subset of neurons with cytosolic GCaMP6f, as well as results from Slc1a3-CreER;Lck-GCaMP6f flox retinas (Figure … WebSep 21, 2024 · (A and B) Cre −/− and Cre +/− macrophages were transduced Cyto-GCaMP6f (A) or Mito-GCaMP6f (B) and then incubated with CellVue Claret-labeled ACs. Confocal microscopy was used to capture time-lapse images every 5 min, with measurements beginning when an AC fell into the plane of focus of a macrophage in the … WebPlasmid pGP-CMV-GCaMP6f from Dr. Douglas Kim's lab contains the insert GCaMP6f and is published in Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354. This … how to save the gmail icon to my desktop