Hantaan virus 76-118 plaque assay

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WebFig. 1. Dose-response curve for Hantaan virus. Serial two-fold virus dilutions were assayed in triplicate cultures, and the number of plaques per well of each virus dilution was … WebJan 1, 1984 · Hantaan virus, strain 76–118, has been implicated as the etiologic agent of Korean hemorrhagic fever largely due to isolation of this virus from rodents in an endemic disease area and serological reactivity using convalescent human sera (4). Consequently, it was of interest to compare this rodent virus to the human isolate (Lee strain).

Cell fusion by haemorrhagic fever with renal syndrome (HFRS

WebOct 5, 2013 · The results indicated the potential of using a pseudotyped lentivirus as a delivery vector for a hantavirus vaccine immunogen. Introduction Hantaviruses constitute a genus of the family Bunyaviridae. Hantaviruses are spherical, enveloped viruses with a genome consisting of three segments of single-stranded, negative-sense RNA. WebMay 6, 2024 · A plaque assay can be used to enumerate viruses that lyse their host cells. In a plaque assay the host cells and virus are incubated together for a short time to allow … nudge you meaning

Identification of a cell surface 30 kDa protein as a ... - ResearchGate

WebHantaan Virus Strain 76 118, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, … WebHantaan virus 76-118 Taxonomy ID: 11602 (for references in articles please use NCBI:txid11602) current name Hantaan virus 76-118 equivalent: Hantaan virus … WebThe assay was subsequently evaluated using a clinical cohort of 50 orthohantavirus patients. Orthohantavirus infection was confirmed in all 50 patients, and 47/50 (94%) … nudge your workplace app

Serological evidence of Hantaan-related viruses in Hong Kong

Characterization of the nucleocapsid protein of Hantaan …

WebJul 18, 2024 · Hantaan virus (HTNV) and Seoul virus ... The viral stocks of 84FLi and L99 were titred three times by semi-log10 dilutions by plaque forming assay. The same virus stocks were used in MNT. Then serial 4-fold dilutions of human sera (starting at 1:20) were incubated with 100 PFU of 84FLi or L99 at the final volume of 100 μl at 4 °C overnight ... WebAutomated immunohistochemical staining was performed using the polyclonal anti-N protein antibody of Hantaan virus 76-118 (kindly provided by BEI Resources; dilution, 1:200) on the Benchmark XT platform (BenchMark XT; Ventana Medical Systems, Tucson, AZ) with cell conditioning for 80 minutes, pre-peroxidase inhibition, and primary antibody … nudge youtubeWebJan 1, 1987 · Four serotypes of hantaviruses have been recognized to date: the prototype Hantaan 76-118 virus and related strains; the urban rat strains which include the Seoul … ninety and nine

"WebJul 18, 2024 · Virus titration by plaque forming assay. a Hantaan virus strain 84FLi; b, Seoul virus strain L99. Microneutralization test. ... 2002; 76 (24):13001–13014. doi: 10.1128/JVI.76.24.13001-13014.2002. [PMC free article] [Google Scholar] 35. Liang MF, Song G, Hang CS, et al. Preliminary study on structure protein of epidemic hemorrhagic … " - Hantaan virus 76-118 plaque assay

Hantaan virus 76-118 plaque assay

Comparison of serological assays to titrate Hantaan and Seoul ...

WebJan 30, 2003 · Plaque reduction neutralization assays indicated that HV114 is closely related to prototype Hantaan (HTN) virus, strain 76-118, which was originally isolated … WebHantaan orthohantavirus (HTNV) is an enveloped, single-stranded, negative-sense RNA virus species of Old World Orthohantavirus. It is the causative agent of Korean …

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WebHantaan virus is the causative agent of hemorrhagic fever with renal syndrome (HFRS). The Hantaan virus strain, Korean hemorrhagic fever virus clone-5 (KHF5), causes weight loss and renal hemorrhage in laboratory mice. Clone-4 (KHF4), which has a single E417K amino acid change in its glycoprotein, is an avirulent variant. In this study, KHF4 and …

WebThis chapter describes four fluorescence-based PCR assays to detect the Crimean-Congo Hemorrhagic Fever (CCHFV), Andes (ANDV), Hantaan (HANV), and Sandfly Fever Sicilian (SFSV) Viruses. These assays are based on species-specific hydrolysis probes targeting the nucleocapsid protein gene for CCHFV and SFSV and the glycoprotein gene for … WebThis method was simpler and more rapid than the ordinary plaque formation method or that of counting infected cell foci by IFA or immunoenzyme assay. In addition, this method may also be applicable in the detection of other enveloped viruses which do not cause a typical cytopathic effect. ... Analysis of Hantaan virus RNA: Evidence for a new ...

WebJul 19, 2024 · Hantaan virus (HTNV), the causative pathogen of hemorrhagic fever with renal syndrome (HFRS), is a negative RNA virus belonging to the Orthohantaviridae … Webstrains of Hantaan virus, including strain 76-118, was at variance with some of the previously measured virus properties. This led us to use buoyant density centrifugation …

WebHantaan virus, isolated near the Hantaan River in Korea, was discovered to be the cause of Korean hemorrhagic fever, after more than 25 years of searching for the etiologic …

WebApr 6, 2024 · On the basis of HTNV strain 76-118, we performed multiple sequence alignments of the hotspots with those of 148 variants with ClustalX2.1 (Conway Institute UCD Dublin, Ireland). The alignment results were also analyzed on WebLogo ( http://weblogo.berkeley.edu/logo.cgi, accessed on 3 December 2024) [ 21 ]. nudge yourselfWebNational Center for Biotechnology Information nudging examples in businessWebMar 4, 2016 · The PRNT 50 antibody titer was estimated as the highest serum dilution that reduced the standard viral dose (Hantaan virus strain 76–118) by 50%. Neutralizing titers of 1:10 or greater were regarded as positive. IFA was performed with Hantaan virus (strain 76–118)-infected Vero E6 cells. nudging ethicsWebFeb 19, 2016 · Yoo, Y. C. et al. Comparison of virulence between Seoul virus strain SR-11 and Hantaan virus strain 76–118 of hantaviruses in newborn mice. Microbiol Immunol 37, 557–562 (1993). nudging effectWebWe characterized the antigenic sites on the nucleocapsid protein (NP) of Hantaan virus (HTN) using 10 monoclonal antibodies (MAbs). At least seven antigenic sites were … nudging financial behaviourWebVirus, cells, and media. Hantaan virus strain 76-118 was obtained at the third A549 cell culture passage from Dr G. R. French, US Army Research Institute of Infectious … ninety and nine burl ivesWebMay 1, 2002 · A polyclonal antibody against the 30K protein blocked binding of Hantaan virus to Vero-E6 cells and, consequently, virus infection. Blocking with the anti-30K antibody reduced virus... ninety and twenty-three hundredths