WebHIF-1α, NF-κB, and iNOS were measured by immunofluorescence of aortic tissues that were previously fixed with PHEMO buffer (68 mM PIPES, 25 mM, HEPES, pH 6.9, 15 mM EGTA, 3 mM MgCl2, 10% [v/v] dimethyl sulfoxide containing 3.7% formaldehyde and 0.05% glutaraldehyde) and were processed by imumunofluoresence double labeling with anti … Web18 de mar. de 2024 · Membranes were blocked with 5% milk powder in 1× PBS-T for 1 h and then incubated overnight at 4 °C with a mouse anti-HIF-1α monoclonal antibody in …
ASAP3 is a downstream target of HIF-1α and is critical for progr
Web11 de abr. de 2024 · The following antibodies were used: p53, PHD2, MMP2, HIF-1α, MRP1, and GAPDH, and the secondary antibody (detailed in Supplementary Table S1). Quantitative RT-PCR: In order to further compare the mRNA transcription levels of 3d-ACS and 2d cultured GC cells, the total RNA in the released cells was extracted using Trizol … WebHIF-1α Antibody detects endogenous levels of total HIF-1α protein. The antibody does not cross-react with other family members at physiological conditions, and does not detect significant levels of hydroxylated HIF-1α. … solhealth
Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody AF1935: R&D …
Web5 de jan. de 2024 · HIF1a Antibody (H1alpha 67) is a mouse monoclonal IgG 2b κ, cited in 183 publications, provided at 200 µg/ml. raised against amino acids 432-528 of HIF-1α of human origin. Anti-HIF1a Antibody (H1alpha 67) is recommended for detection of HIF-1α of mouse, rat and human origin by WB, IP, IF and IHC (P); also reactive with additional … Web8 de abr. de 2024 · The percentage of positive tumour cells was scored for each antibody, using only nuclear staining for HIF-1α, and only membranous staining for CA-IX. Cut-off … WebHIF-1α Antibody (C-19) has been replaced by a more specific monoclonal antibody, HIF-1α (28b) that provides a stronger signal & more reproducible data. Choose a Store Santa … sma exemption hawaii